Fig. 4.
Inhibition of androgen-independent prostate tumor growth by mAb F77. Male nude mice were injected s.c. with 1 × 106 PC3 on the right flank (A) and 0.5 × 106 A431 on the left flank (B). Treatment started at day 7 after tumor cell injection. mAb F77 was administered by i.p. injection of 200 μg per dose at days 7 and 9 and 100 μg per dose at day 11 and 13. PBS solution was used as the vehicle control. Mice have to be killed when tumors are greater than 1 cm in diameter in accordance with the University of Pennsylvania Institutional Animal Care and Use Committee. Du145 1 × 106 (C) and 2 × 106 (D) were transplanted onto male nude mice. mAb F77 or a control mouse IgG was administered at day 7 by i.p. injection of 200 μg per dose every other day for four times in total (C). Posttreatment (D) started at day 11 when tumor volume was greater than 30 mm3. The data are presented as mean tumor volume (in mm3) ± SEM; P ≤ 0.01.