Fig. 3.

Cdh1 promotes PFKFB3 degradation in the nucleus. (A) Cdh1 overexpression promoted the degradation of coexpressed rat PFKFB3 cDNA (RB2K6 splice variant), but did not affect its KEN-box-mutant form (PFKFB3mut). (B) In Cdh1-overexpressing cells, coexpressed rat PFKFB3 (RB2K6 splice variant) was present only in the nucleus, whereas its KEN-box-mutant form (PFKFB3mut) accumulated in the nucleus and was spread throughout the cytosol. (C) Serum-induced exit from quiescence promoted PFKFB3 accumulation, both in the nucleus and in the cytosol, as well as a decrease in Cdh1 in the nucleus. The detected protein in A and C was quantified by densitometry from three independent experiments.