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. 2009 Dec 22;107(2):821–826. doi: 10.1073/pnas.0909235107

Fig. 3.

Fig. 3.

Impaired IgE production in NFIL3 KO B cells. (A) B cells from WT and NFIL3 KO mice were stimulated with LPS/IL-4 for 5 days, and cell intracellular expression of IgE and IgG1 was examined by flow cytometry. Two experiments were performed with similar results. (B) Ig levels secreted by B cells stimulated for 5 days with LPS (IgM, IgG2a, IgG2b, and IgG3) or LPS/IL-4 (IgE and IgG1) were determined by ELISA (n = 3; *P = 0.0024). (C) Reduced GLε and PSTε in NFIL3 KO B cells. Cells were unstimulated (open bars) or stimulated with LPS alone (gray bars) or LPS/IL-4 (closed bars) for 3 and 5 days, after which RNA was prepared. Germ-line transcript (GLT) and PST were analyzed by real-time RT-PCR. Relative expression of GLT and PST was normalized by the expression of Hprt1 mRNA. The expression of RNA in the unstimulated WT cells was set as 1. The graphs represent means and SD from the three WT mice and three NFIL3 KO mice (*P < 0.005, GLε expression between WT and KO B cells stimulated with LPS/IL-4 for 3 days; **P < 0.02, PSTε expression between WT and KO B cells stimulated with LPS/IL-4 for 5 days).