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NFIL3 binds Iε promoter in vivo. M12NFIL3 cells were unstimulated or stimulated with LPS/IL-4, and soluble chromatin complex was applied for ChIP by anti-NFIL3 and control antibodies. NFIL3 binding to the indicated genes in the coprecipitated DNA was assessed by real-time PCR. The relative enrichment by control IgG in unstimulated cells was set as 1. The averages of relative NFIL3 binding activity from three experiments are indicated (*P = 0.06, **P =0.03, ***P < 0.01).
