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. 2009 Dec 22;107(2):832–837. doi: 10.1073/pnas.0913170107

Fig. 3.

Fig. 3.

Atg7−/− erythroid cells have a decreased lifespan and a significantly increased susceptibility to cell death in both blood and spleen. (A) qPCR amplification of Beclin1, Atg5, and Atg7 cDNA in the indicated BM erythroid developmental stages from 8-week-old WT mice. (B) Survival of CFSE-labeled WT and Vav-Atg7−/− red blood cells in WT hosts (*P < 0.05). This experiment was repeated three times with similar results. (C) Annexin V levels in peripheral red blood cells ex vivo, after 6 and 24 h of culture. The percentage of Annexin V+ cells was determined by gating on live cells and is shown in the left panel. Bar heights indicate total percentage of Annexin V+ cells (interaction: *P < 0.05, two-way ANOVA, n = 3). (D) Representative histograms of active caspase 3 levels on spleen erythroid stages from 9-week-old mice, gated as in Fig. 2A (*P = 0.0159, n = 4).