ddlc1 mutants accumulate Ref(2)p in muscles and have decreased numbers of synaptic boutons. (A–E) Ref(2)P staining (green) of third instar larval muscles of (A) WT (n = 8 animals), (B) ddlc1 mutant (n = 8 animals), (C) ddlc1 mutant larvae expressing AR Q52 in the presence of dihydroxytestosterone (DHT; n = 8 animals), (D) ddlc1 RNAi (Ddlc1-IR) expression by the muscle c57 GAL4 driver (n = 5 animals), and (E) ddlc1 RNAi (Ddlc1-IR) expression by the motor neuron d42 GAL4 driver (n = 5 animals). (F–H) Quantification of bouton numbers in neuromuscular junctions. (F) WT (n = 9 animals) and (G) ddlc1 mutant (n = 11 animals) stained with HRP antibody. (H) Average number of boutons (±SEM) in the genotypes shown in F and G. *P < 0.00001, significant difference from control.