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. 2009 Dec 22;107(2):742–747. doi: 10.1073/pnas.0907967107

Fig. 5.

Fig. 5.

ddlc1 mutants accumulate Ref(2)p in muscles and have decreased numbers of synaptic boutons. (A–E) Ref(2)P staining (green) of third instar larval muscles of (A) WT (n = 8 animals), (B) ddlc1 mutant (n = 8 animals), (C) ddlc1 mutant larvae expressing AR Q52 in the presence of dihydroxytestosterone (DHT; n = 8 animals), (D) ddlc1 RNAi (Ddlc1-IR) expression by the muscle c57 GAL4 driver (n = 5 animals), and (E) ddlc1 RNAi (Ddlc1-IR) expression by the motor neuron d42 GAL4 driver (n = 5 animals). (F–H) Quantification of bouton numbers in neuromuscular junctions. (F) WT (n = 9 animals) and (G) ddlc1 mutant (n = 11 animals) stained with HRP antibody. (H) Average number of boutons (±SEM) in the genotypes shown in F and G. *P < 0.00001, significant difference from control.