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. 2009 Dec 22;107(2):680–685. doi: 10.1073/pnas.0908302107

Fig. 3.

Fig. 3.

Msh2-Msh6 moves from 2-Ap:T to G:T without dissociating from DNA. (A) Fluorescence anisotropy shows Msh2-Msh6 binding mismatched 2-Ap:T/7 bp/G:TTAMRA and matched 2-Ap:T/7 bp/G:CTAMRA DNA rapidly (kON = 1.35 and 1.4 × 107 M-1 s-1, respectively). (B) 2-Ap fluorescence yields the same rate for 2-Ap:T/7 bp/G:CTAMRA, but 2-Ap:T/7 bp/G:TTAMRA shows biphasic kinetics. (C) 2-Ap fluorescence reports rapid release of Msh2-Msh6 from 2-Ap:T in 2-Ap:T/7 bp/G:TTAMRA with an external trap (unlabeled G:T) or with only buffer added after 0.25 s (kOFF = 0.8–1.2 s-1). (D) Corresponding TAMRA anisotropy shows slow release of Msh2-Msh6 from 2-Ap:T/7 bp/G:TTAMRA (kOFF = 0.044 s-1).