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. Author manuscript; available in PMC: 2010 Aug 15.
Published in final edited form as: J Immunol. 2009 Jul 22;183(4):2267. doi: 10.4049/jimmunol.0802960

Figure 3.

Figure 3

DNMT3a KO T cells spontaneously secrete IFN-γ and IL-4 in non-selected culture in vitro. (A) ELISA for IL-2 (top left), IFN-γ (top right), and IL-4 (bottom) of cell culture supernatant harvested from primary stimulation or after the indicated number of weeks of culture with weekly stimulation with anti-CD3, irradiated splenocytes, and IL-2 as described in Materials and Methods. Results are representative of two experiments. (B) Flow cytometry depicting IL-4 versus IFN-γ intracellular staining of CD4 T cells from DNMT3a WT (top) or KO (bottom) mice. The panel depicts cells harvested after 5 weeks of culture. Results are representative of three experiments.