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. Author manuscript; available in PMC: 2010 Feb 10.
Published in final edited form as: Cell Stem Cell. 2008 Mar 6;2(3):241. doi: 10.1016/j.stem.2008.01.002

Figure 5. Differentiation of SIRT1−/− mES cells cultured in absence of 2-ME.

Figure 5

SIRT1−/− mES cells were passaged one time in absence of 2-ME. Alkaline phosphatase was stained as described in Materials and Methods. SSEA-1 and Oct-4 were measured by flow cytometry.