Skip to main content
. Author manuscript; available in PMC: 2011 Feb 9.
Published in final edited form as: Biochemistry. 2010 Feb 9;49(5):951. doi: 10.1021/bi9017335

Figure 4.

Figure 4

A) Highlighted regions of 15N-1H HSQC spectra of par3-PDZ3 saturated with VE-Cad peptide (MLAELYGSDPQEELIIcoo-) shown in gray, overlaid with spectra saturated with mutant VE-Cad peptide (MLAELYGS(A)PQEELIIcoo-) shown in red. B) Chemical shift difference in par3-PDZ3 calculated from Δδppm = [(5*ΔδHN)2 + (ΔδN)2]1/2, where ΔδHN and ΔδN represent chemical shift differences between the two saturated forms of par3-PDZ3. All residues with Δδppm > 0.09 are shown. C) Par3-PDZ3 chemical shift map resulting from D777A binding relative to wt-VE-Cad binding. Highlighted residues indicate changes in the electronic environment for a region along the β2-strand consistent with a disruption of distal contact.