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Forward- and reverse-SILAC combined with LC-MS/MS for the comparative analysis of protein expression in HL-60 cells upon arsenite treatment (A). Shown in (B) and (C) are a summary of the number of proteins quantified from three independent SILAC experiments and the distribution of expression ratios (treated/untreated) for the proteins quantified, respectively. “Set 1” and “Set 2” results were obtained from forward, and “Set 3” results were from reverse SILAC experiments.
