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. Author manuscript; available in PMC: 2011 Feb 3.
Published in final edited form as: J Am Chem Soc. 2010 Feb 3;132(4):1194. doi: 10.1021/ja907778b

Figure 2.

Figure 2

(a) Confocal fluorescence ratio images of live C6 rat glioma cells grown in basal media and stained with 2 μM RCS1 for 10 min at 37 °C. (b) C6 cells treated with 1 mM ascorbate for 4 h and stained with 2 μM RCS1 for 10 min at 37 °C. (c) C6 cells from condition (b) treated with 1 mM TEMEA for 5 min by direct addition to the Petri dish on the microscope stage. (d) C6 cells incubated with 200 μM BCS for 4 h and stained with 2 μM RCS1 for 10 min at 37 °C. (e) C6 cells treated with 200 μM BCS and 1 mM ascorbate for 4 h, then stained with 2 μM RCS1 for 10 min at 37 °C. (f) Bar graph representing the integrated intensity from 564-639 nm over the integrated fluorescence intensity from 522-554 nm. Values are the mean ratio generated from the intensity from five randomly selected fields. Error bars represent standard error measurement (s.e.m.)