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SNF was detected by measuring the fluorescent intensity of HEK293 cells using a flow cytomtry. A: The background was 2.45%. The percentages of green fluorescent cells were 99.21%(B), 99.22%(C), 98.65%(D) and 99.39%(E) for group A to D respectively on Day 7 after the treatment. Fluorescence intensity of infected HEK 293 cells was inversely proportional to SNF level. SNF against Ad-EGFP-MDR1 was not detected in all groups.
