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. Author manuscript; available in PMC: 2011 Feb 1.
Published in final edited form as: Anal Chem. 2010 Feb 1;82(3):1082. doi: 10.1021/ac902456n

Figure 4.

Figure 4

Large fluorescent RNA generated by transcription in the presence of CTP and tCTP at different concentrations. The top row of A and B shows the 1.2 % agarose gel exposed by UV light prior to staining with ethidium bromide (EtBr). The CTP and tCTP concentrations are provided below the images. The reaction in the lane next to the marker contained DNA template, primers, enzyme but no CTP or tCTP. A) 827 nucleotide RNA obtained from transcription of the Borrelia miyamotoi flagellin protein gene. B) 207 nucleotide long B12 riboswitch of E. coli. C) Reverse transcription of unlabeled or tC labeled RNA. The products of the reverse transcription reactions were analyzed on a 1.4 % agarose gel and visualized based on the incorporation of [α-32P]dTTP. Unlabeled B12 riboswitch (lane 1); 100 % tC labeled B12 riboswitch (lane 2); unlabeled Borrelia RNA (lane 3); 100 % tC labeled Borrelia RNA (lane 4); reaction as in lane 1 but without enzyme (lane 5).