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. Author manuscript; available in PMC: 2011 Jan 1.
Published in final edited form as: J Mol Neurosci. 2009 Aug 12;40(1-2):21. doi: 10.1007/s12031-009-9248-x

Figure 2.

Figure 2

Differentiation of the NMJ in the absence of the γ-subunit. High magnification views of a single endplate from wholemount diaphragm muscle at E18.5, double-labeled with synaptotagmin-2 (Syt-2) antibody (green) and Texas-red conjugated α-bungarotoxin (red). Individual endplate (AChR cluster) in the mutant (e, arrow) appeared less intensely labeled by α-bungarotoxin, but their sizes are bigger, compared to the wild type (b, arrow). In both WT and mutant muscles, Syt-2 antibody staining was highly concentrated at the nerve terminals (arrowheads in a, d). Scale bar 10 µm