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. Author manuscript; available in PMC: 2010 Feb 10.
Published in final edited form as: Diabetes. 2006 Jan;55(1):148.

FIG. 4.

FIG. 4

Knockdown of ATGL in 3T3-L1 adipocytes. siRNA-mediated knockdown of ATGL in 3T3-L1 adipocytes was achieved by electroporating 3T3-L1 adipocytes with either control siRNA (Cont) or ATGL-specific siRNA (ATGL) at day 4 of differentiation (three experiments, n = 3 each). ATGL expression (A), basal (B) and stimulated (C) glycerol release, and basal (D) and stimulated (E) NEFA release were then evaluated at day 6 of differentiation. Gene expression was normalized to 18S ribosomal RNA and expressed relative to ATGL expression in the control group. Glycerol and NEFA release were normalized to total cellular protein and expressed relative to the nonstimulated control group. Iso, isoproterenol. ***P < 0.001; ****P < 0.0001.