Hn1 is expressed in murine and human glioma cell lines. (a) Northern blot analysis of Hn1 mRNA in GL261 cells. The migration of the 18 S rRNA is indicated. (b) Western blot analysis of HEK293 cells (HEK), HEK293 cells transfected with a recombinant murine Hn1 protein (HEK-Hn1), murine B16.F10 and GL261 cells, and the human U118 and U87 glioma cells using the rabbit polyclonal anti-Hn1 antibody. (c) Western blot analysis, using the anti-Hn1 antibody, show Hn1 expression in GL261 cells transduced at an M.O.I. of 5,000 with either Hn1-siRNA AAV6 (siRNA) or control H1-AAV6 (H1-control)