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. 2010 Feb 10;5(2):e9156. doi: 10.1371/journal.pone.0009156

Figure 3. Fluorescence of 3D7HT-GFP throughout the life cycle.

Figure 3

GFP fluorescence was observed in different life stages of the parasite. Parasites were counterstained with DAPI nuclear dye (blue) (VectorLabs, UK). Fluorescent and DIC images are shown alongside each other, or as an overlay. (A) liver stage trophozoites in a primary human hepatocyte (B) blood ring stage, (C) trophozoites, (D) schizont, (E) male gametocyte, (F) female gametocyte, (G) ookinete obtained 29 hours post-feed in infected An. stephensi blood meal, (H) an infected gut 8 days post-feed, (I) 10 day oocyst, (J) infected salivary gland dissected 18 days post-feed, (K) salivary gland sporozoite. White scale bar 5 µm, orange scale bar 50 µm.