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. Author manuscript; available in PMC: 2011 Jan 1.
Published in final edited form as: J Reprod Immunol. 2009 Nov 27;84(1):24. doi: 10.1016/j.jri.2009.10.006

Figure 3.

Figure 3

Expression of ZP3 protein in AH109 (pAS2-1-ZP3). The expression of ZP3 protein in AH109 was confirmed using anti-ZP3 antibody. (A) Anti-ZP3 antibody specifically recognized a protein band of ~53 kD in AH109 culture extract (lane a). The antibody recognized the expected protein band of 42 kD in HepG-2 lysate that was used as a positive control, supplied by the manufacturer (lane b). The antibody did not react with any protein band in vector (pAS2-1) alone culture lysate without ZP3 (lane c). (B) Control rabbit immunoglobulins did not react with any band in AH109 culture extract (lane a), HepG-2 lysate (lane b), or pAS2-1 vector alone culture extract (lane c).