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Confirmation of ZP3 insert cloned into mammalian pM vector (3.5 kb) at EcoRI and BamHI sites. The pM-ZP3 mammalian clone digested with EcoRI (lane b) or BamHI (lane c) alone did not show, but the clone digested with both the EcoRI and BamHI restriction enzymes (lane d), showed the expected 1 kb ZP3 band in the agarose gel electrophoresis. The DNA molecular weight ladder has been included for comparison (lane a). The authenticity of the clone was further confirmed by sequencing.
