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. Author manuscript; available in PMC: 2011 Jan 1.
Published in final edited form as: J Reprod Immunol. 2009 Nov 27;84(1):24. doi: 10.1016/j.jri.2009.10.006

Figure 6.

Figure 6

Confirmation of the six interacting clones (GS-2, GS-6, GS-7, GS-12, GS-18, and Col99) in the pVP-16 mammalian vector digested with various restriction enzymes. The undigested pVP-16 vector (lane a), pVP-16 digested with HindIII and SalI (lane b), pVP-16-GS-2 clone digested with PstI and XbaI (lane c), pVP-16-GS-6 clone digested with HindIII and PstI (lane d), pVP-16-GS-7 clone digested with Hind III and SalI (lane e), pVP-16-GS-12 clone digested with HindIII and SalI (lane f), pVP-16-GS-18 clone digested with HindIII and EcoRI (lane g), and pVP-16-Col99 clone digested with HindIII and SalI (lane h). The DNA molecular weight ladder has been included for comparison (lane i). The authenticity of the clones was further confirmed by sequencing.