Table 1.
Clone | Sequence of primers | Restriction sites | Size of amplicon |
---|---|---|---|
GS-2 | Sense: 5’AAAACTGCAGTGGCAGTAGCCTGGGC 3’ Anti-sense: 5’GCTCTAGATTACTGGAGGTGACTGAA 3’ |
PstI XbaI |
652 bp |
GS-6 | Sense: 5’AAAACTGCAGCGGGAGCTACAAGCATG 3’ Anti-sense: 5’CCCAAGCTTCTAAAAATTCAAAAAAA 3’ |
PstI HindIII |
228 bp |
GS-7 | Sense: 5’ ACGCGTCGACTGTTGTTGGCCCTGGT 3’ Anti-sense: 5’CCCAAGCTTCTAGAAGTCACAGCCATC 3’ |
SalI HindIII |
440 bp |
GS-12 | Sense: 5’ ACGCGTCGACCACACACAGTCTTTGC 3’ Anti-sense: 5’ CCCAAGCTTTTATTCTTTCAGGAAA 3’ |
SalI HindIII |
470 bp |
GS-18 | Sense: 5’ACGCGTCGACGCTCCCTGGATGGCT 3’ Anti-sense: 5’ GCTCTAGATCACTTTCTCACAGCC 3’ |
SalI XbaI |
326 bp |
Col99 | Sense: 5’ ACGCGTCGACACGAGAAGCACCTACT 3’ Anti-sense: 5’CCCAAGCTTTTAAGGAACCGAGAT 3’ |
SalI HindIII |
479 bp |