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. Author manuscript; available in PMC: 2011 Jan 1.
Published in final edited form as: J Reprod Immunol. 2009 Nov 27;84(1):24. doi: 10.1016/j.jri.2009.10.006

Table 1.

Sequence of primers used for PCR-amplification of the interacting clones.

Clone Sequence of primers Restriction sites Size of
amplicon
GS-2 Sense: 5’AAAACTGCAGTGGCAGTAGCCTGGGC 3’
Anti-sense: 5’GCTCTAGATTACTGGAGGTGACTGAA 3’
PstI
XbaI
652 bp
GS-6 Sense: 5’AAAACTGCAGCGGGAGCTACAAGCATG 3’
Anti-sense: 5’CCCAAGCTTCTAAAAATTCAAAAAAA 3’
PstI
HindIII
228 bp
GS-7 Sense: 5’ ACGCGTCGACTGTTGTTGGCCCTGGT 3’
Anti-sense: 5’CCCAAGCTTCTAGAAGTCACAGCCATC 3’
SalI
HindIII
440 bp
GS-12 Sense: 5’ ACGCGTCGACCACACACAGTCTTTGC 3’
Anti-sense: 5’ CCCAAGCTTTTATTCTTTCAGGAAA 3’
SalI
HindIII
470 bp
GS-18 Sense: 5’ACGCGTCGACGCTCCCTGGATGGCT 3’
Anti-sense: 5’ GCTCTAGATCACTTTCTCACAGCC 3’
SalI
XbaI
326 bp
Col99 Sense: 5’ ACGCGTCGACACGAGAAGCACCTACT 3’
Anti-sense: 5’CCCAAGCTTTTAAGGAACCGAGAT 3’
SalI
HindIII
479 bp