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. Author manuscript; available in PMC: 2010 Feb 10.
Published in final edited form as: J Immunol. 2006 Dec 1;177(11):7723. doi: 10.4049/jimmunol.177.11.7723

FIGURE 3.

FIGURE 3

Serum IgG1 and IgG2a titers as well as the frequency of splenic PCs produced by transferred Tg λ B cells are reduced early on in the humoral immune response. Tg κ or λ cells were adoptively transferred and recipients immunized with NP, NIP, or CFA alone (naive). Serum and spleens from recipients were collected at days 5, 7, and 10 following challenge. Left panels show total levels of Tg NP-specific serum IgG1, IgG2a, and IgM as measured by ELISA using allotype-specific detection mAbs. Right panels show splenic Tg anti-NP IgG1, IgG2a, and IgM PCs quantified by ELISPOT from the same recipients. The data shown are mean ± SD (n = 3) and are representative of three experiments.