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. Author manuscript; available in PMC: 2010 Apr 1.
Published in final edited form as: Dev Cell. 2009 Oct;17(4):494. doi: 10.1016/j.devcel.2009.08.008

Figure 2. Expression Pattern of lat-1 and lat-2.

Figure 2

(A) lat-1 mRNA in early blastomeres and (B) the gonad detected by in situ hybridization. (C and D) Zygotic expression of lat-1 in early embryonic development.

(C) Expression of a lat-1p::gfp reporter gene at 26-cell stage. AB descendants are labeled “alaa” for ABalaa, etc.

(D) Membrane localization of a rescuing in-frame LAT-1::GFP fusion protein, 200-cell stage.

(E and F) Expression of lat-1 and lat-2 overlaps in the pharynx primordium. (E) lat-1p::gfp expression with strong signal in the pharyngeal primordium (“P”) and ventral epidermal precursors (“E”). (F) lat-2p::gfp expression at the same stage is confined to the pharynx primordium.

(G and H) Asymmetric expression of lat-1 in epidermal precursors during dorsal intercalation visualized with a lat-1p::gfp transgene (G) or by detection with an α-LAT-1 antibody. Arrows indicate position left seam cells, and arrowheads mark LAT-1-positive intercalating left dorsal epidermal cells. Scale bars in (C), (G), and (H) = 10 μm, in (D), (E), and (F) = 5 μm.