Figure 5. Cell Fate Transformations in Latrophilin Mutants.

(A) MSap daughters and ABala granddaughters expressing lag-2p::gfp reporter gene in WT embryo.

(B) lat-1 mutants typically express the lag-2p::gfp reporter gene in a WT configuration.

(C) In a minority of lat-1 embryos, expression of lag-2p::gfp in the ABal lineage is absent or displaced. Lateral views, anterior to the left.

(D–F) α-3NB12 immunostaining of terminally differentiated pharyngeal muscle cells after pharynx elongation. Arrows indicate the terminal bulb. (D) WT larva, 21 3NB12⁺ cells. (E) lat-1 mutant larva with abnormal pharynx morphology and 25 3NB12⁺ cells; the overall structure of the pharynx is preserved, but 3NB12⁺ cells are displaced and extra cells are found in ectopic positions. (F) Mutant larva with a normal number of 21 3NB12⁺ cells. The pharynx is disorganized and pharyngeal morphogenesis has failed. All scale bars = 5 μm.