Table 1.
Frequency of Embryonic and Larval Arrest Phenotypes Depending on Maternal and Zygotic Genotype for lat-1 and lat-2
| Strain/Mating | Maternal Genotype | Zygotic Genotype | Embryonic Arrest [%] | L1 Arrest [%] | N (Sets) |
|---|---|---|---|---|---|
| N2 | +/+ | +/+ | 0.6 ± 0.4 | 0.6 ± 0.6 | 1513 (5) |
| lat-1(ok1465) | −/− | −/− | 16.8 ± 2.5 | 55.5 ± 2.4 | 1789 (8) |
| lat-2(tm463) | −/− | −/− | 0.5 ± 0.5 | 3.6 ± 3.6 | 395 (3) |
| lat-1(ok1465) lat-2(tm463) | −/− | −/− | 24.2 ± 3.3 | 73.4 ± 3.4 | 1026 (4) |
| lat-1(ok1465)/mIn1[mIs14 dpy-10(e128)] | +/− | mendelian +/+, +/−, −/− | 1.0 ± 0.2 | 26.3 ± 0.8 | 1004 (6) |
| lat-1(ok1465) mated to WT males | −/− | +/− | 5.6 ± 0.6 | 1.5 ± 0.8 | 1058 (3) |
| lat-1(ok1465) lat-2(tm463) mated to mIn1 males | −/− | ++/−− | 11.9 ± 0.9 | 4.5 ± 0.9 | 1096 (3) |
| mom-2(t2180ts) (25°C) | −/− | −/− | 99.7 ± 0.1 | 0.3 ± 0.1 | 2078a (3) |
| mom-2(t2180ts) (15°C) | −/− | −/− | 4.6 ± 0.6 | 1.7 ± 0.5 | 2028a (3) |
| lat-1(ok1465); mom-2(t2180ts) (15°C) | −/−;−/− | −/−;−/− | >95.0 | <5.0 | 1209a (2) |
| lat-1(ok1465) lat-2(tm463); mom-2(t2180ts) (15°C) | −/−;−/− | −/−;−/− | >98.0 | <2.0 | 872a (2) |
Line 5 shows the numbers for the offspring of heterozygous hermaphrodites carrying a balancer chromosome with an expected Mendelian frequency of 25% for homozygous mutants. Preselection of homozygous larvae by lack of balancer chromosome leads to indistinguishable results. Experimental spread is indicated as SEM.
a
mom-2(t2180ts) numbers obtained from pooled progeny of three mothers per plate.