Table 2.
Summary of AB and MS Spindle Alignment Defects in Latrophilin Mutants
| Strain (Incubation Temperature) | Maternal Genotype | Zygotic Genotype | ABal Spindle Defect [% (n/Total)] | MS Orientation Delay [% (n/Total)] | ABalxx Delay [% (n/Total)] | ABalxx Defect [% (n/Total)] |
|---|---|---|---|---|---|---|
| N2 (25°C) | +/+ | +/+ | 0 (0/6) | 0 (0/6) | 0 (0/6) | N.A. |
| N2 (20°C) | +/+ | +/+ | 0 (0/12) | 0 (0/12) | 0 (0/12) | N.A. |
| lat-1(ok1465)/mIn1 [mIs14 dpy-10(e128)] (25°C) | +/− | +/+, +/− | 0 (0/10) | N.D. | N.D. | N.D. |
| lat-1(ok1465)/mIn1 [mIs14 dpy-10(e128)] (25°C) | +/− | −/− | 0 (0/5) | N.D. | N.D. | N.D. |
| lat-1(ok1465); Ex[lat-1(+)] (25°C) | −/−; Ex[lat-1(+)] | −/−; Ex[lat-1(+)] | 75 (3/4)a | N.D. | N.D. | N.D. |
| lat-1(ok1465) (25°C) | −/− | −/− | 100 (17/17) | N.D. | N.D. | N.D. |
| lat-1(ok1465) lat-2(tm463) (25°C) | −/− | −/− | 100 (14/14) | N.D. | N.D. | N.D. |
| lat-1(ok1465) mated to WT male (25°C) | −/− | +/− | 100 (4/4) | N.D. | N.D. | N.D. |
| lat-1(ok1465) (20°C) | −/− | −/− | 75 (42/56) | 59 (10/17) | 47 (7/15) | 33 (5/15) |
| lat-1(ok1465) lat-2(tm463) (20°C) | −/− | −/− | 67 (10/15) | 60 (9/15) | 53 (8/15) | 27 (4/15) |
N.D., not determined; N.A., not applicable.
a
2/4 animals hatched. This number is an underestimate of the ABal spindle defect rescuing capacity of Ex[lat-1(+)] due to array loss and low transgene expression in the maternal germline.