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. Author manuscript; available in PMC: 2010 Feb 10.
Published in final edited form as: J Biol Chem. 2007 Mar 27;282(20):14807. doi: 10.1074/jbc.M611550200

FIGURE 2. GPAT1 activity and expression in rat liver.

FIGURE 2

Rats were treated with 1.0–2.0 × 1012 particles/ml of Ad-GPAT1 or Ad-EGFP virus for 5–7 days (Ad-EGFP, n = 9; Ad-GPAT1, n = 11). A, GPAT1 enzyme activity was determined in total liver membranes after inhibiting endoplasmic reticulum GPAT with NEM. Results are expressed as mean ± S.E. B, anti-FLAG Western blot of total membranes from primary hepatocytes infected with Ad-GPAT1 (Control), from liver of a rat infected with Ad-EGFP (EGFP), and from liver, gastrocnemius muscle (2 lanes), and heart (2 lanes) of rats infected with Ad-GPAT.