Table I. Summary of the activities and subcellular distribution of CTIP1 mutants.

Refer to Fig. 3, A and B, for a schematic representation of each mutant. Note that CTIP1-(1–378) and -(1–263) both exhibited disrupted nuclear localization (see Fig. 6, M–P and Q–T, respectively) relative to wild-type CTIP1-(1–776) and the two other truncation mutants (1–601) and 407–776). HEK293 cells were transfected with HA-CTIP1 expression vectors (in the absence of cotransfected Myc-ARP1), stained with appropriate antibodies, and qualitatively scored for the observed distribution pattern (focal, focal and diffuse, diffuse) by a naive observer. The numbers are derived from three independent transfection experiments

CTIP1 mutant	Interaction with ARP1-(1–414) in vitro	Potentiation of ARP1-mediated repression	Subcellular distribution (indirect immunofluorescence/confocal microscopy)
			Foci	Foci/Diffuse	Diffuse	Comments
			observed frequency/number of cells counted
1–776	+	++	96/120	24/120	0/120	Nuclear only
1–601	+	+	0/90	76/90	14/90	Nuclear only
407–776	+	−	0/120	0/120	120/120	Nuclear only
1–378	+	−	0/90	0/90	90/90	Cytoplasmic and nuclear
1–263	−	−	0/90	0/90	90/90	Cytoplasmic and nuclear