Figure 5.

Helix-scanning mutagenesis of connexon43. Each of the four helices was substituted by a poly-alanine helix (shown in green). The coloring scheme is the same as in Figure 4. The M4 substitution is the only one which preserved channel function (Bao et al., 2005), consistent with the assignment of Fleishman et al. (2004) that M4 is on the perimeter of the channel.