Figure 2.
Localization GFP-eIF4AIII in BY-2 tobacco cells. (A) control cells, six nuclei with diffuse signal in the nucleoplasm are shown; (B) mitotic control cell in anaphase: more signal is associated with the separating chromosomes than with the mitotic spindle; (C) cells treated by 5% ethanol for 5 h show localization of signal in nucleoli and speckles. (D) cells treated by MG132 for 5 h show uniform localization in nucleoli only. Bar, 10 µm. Arrows indicate position of nucleoli, arrowheads indicate splicing speckles.