Table 1.
List of differentially phosphorylated consensus peptides
| Consensus | Kinase | sor vs. suc t-test | P up/down |
| ESSYSYEEI | 0.0003 | down | |
| PASPSPQRQ | Cdk5-p23 | 0.0014 | down |
| PKRGSKDG | AGC | 0.0019 | up |
| IREESPPHS | 0.0019 | down | |
| RTPPPSG | MAPK | 0.0020 | down |
| PASQTPNKTt | CDC2 | 0.0029 | down |
| STNEYMDMK | PI3 kinase | 0.0030 | down |
| SEENSKKTV | CKI | 0.0040 | down |
| APTPGGRR | 0.0043 | down | |
| RFTDTRKDE | CaM-III | 0.0046 | down |
| LSELSRRRI | ds-RNA | 0.0062 | up |
| PINGSPRTP | CDC2 | 0.0065 | down |
| TEGQYELQP | Tyr-K | 0.0068 | up |
| KRAQISVRGL | 0.0069 | down | |
| AKRISGKMA | 0.0069 | up | |
| VVGGSLRGA | AGC | 0.0071 | down |
| KRPSNRAKA | 0.0072 | up | |
| ERQKTQTKL | SnRK, MLCK | 0.0073 | down |
| EEGISQESS | 0.0080 | up | |
| PVPEYINQS | EGFR (Tyr-K) | 0.0082 | down |
| FGHNTIDAV | 0.0082 | down | |
| ARVFSVLRE | CaM-II | 0.0085 | down |
| SNDDSDDDD | CKII | 0.0085 | down |
| GGVDYKNIH | Tyr-K | 0.0094 | up |
| SRSRSRSRS | 0.0103 | up | |
| SPSLSRHSS | GSK3 | 0.0107 | down |
| RAKRSGSV | 0.0120 | down | |
| RRASLG | AGC 1/2 | 0.0128 | down |
| GRASSHSSQ | S6K | 0.0129 | down |
| SGYISSLEY | CKII | 0.0139 | down |
| FFRRSKIAV | AGC | 0.0140 | up |
| STNDSPL | beta-ARK | 0.0145 | down |
| LRRASPG | 0.0149 | up | |
| SAVASNMRD | GRK | 0.0154 | up |
| KRPSGRAKA | 0.0160 | up | |
| KRSNSVDTS | AGC | 0.0165 | down |
| RQLRSPRRT | CDC2 | 0.0171 | up |
| GRALSTRAQ | CDPK, PhK | 0.0172 | down |
| VSRTSAVPT | AGC | 0.0173 | down |
| TRKISQTAQ | AGC | 0.0174 | down |
| STTVSKTET | 0.0180 | down | |
| ESPASDEAE | 0.0184 | up | |
| LSYRGYSL | PhK | 0.0185 | down |
| DDINSYEAW | 0.0186 | up | |
| PNVSYIASR | 0.0191 | down | |
| KQPIYIVME | FES (Tyr-K) | 0.0195 | up |
| LVVASAGPT | 0.0198 | down | |
| TGFLTEYVA | MAPKK | 0.0198 | up |
| TEDQYSLVE | Src | 0.0212 | up |
| SSSSSPKAE | MAPK | 0.0213 | up |
| EKAKSPVPK | 0.0221 | down | |
| RRRASVA | AGC1/2 | 0.0221 | down |
| APVASPAAP | MAPK | 0.0225 | down |
| LRRLSTKYR | AGC1/2 | 0.0234 | down |
| EKHHSIDAQ | 0.0256 | down | |
| VRKRTLRRL | SnRK, AGC | 0.0266 | down |
| DLPGTEDFV | GRK2 | 0.0277 | down |
| LSEHSSPEE | CKII | 0.0278 | down |
| KREASLDNQ | AGC | 0.0279 | down |
| TKKQSFKQT | AGC | 0.0280 | up |
| VRLRSSVPG | autoP | 0.0285 | down |
| KRPSLRAKA | 0.0293 | up | |
| PGPQSPGSP | 0.0308 | down | |
| YSGHSMSDP | 0.0309 | up | |
| ADGVYAASG | FES (Tyr-K) | 0.0311 | up |
| ENQASEEED | CKII | 0.0317 | down |
| TLASSFKRR | AGC | 0.0324 | up |
| TVKSSKGGP | AGC | 0.0326 | down |
| GVLRRASVA | 0.0327 | up | |
| SPRKSPRKS | sperm-specific | 0.0328 | down |
| PRRDSTEGF | SnRK, AGC | 0.0332 | down |
| RRRRAASVA | 0.0346 | down | |
| SRKDSLDDS | GRK | 0.0371 | down |
| ENPEYLGLD | Tyr-K | 0.0380 | down |
| KAKTTKKRP | 0.0382 | up | |
| RRPSV | 0.0392 | down | |
| QKAQTERKS | AGC | 0.0401 | down |
| AKAKTTKKR | 0.0404 | up | |
| GSDVSFNEE | CKII | 0.0409 | down |
| DEPSTPYHS | GSK3 | 0.0409 | down |
| SSRPSSNRS | CDPK, AGC | 0.0411 | up |
| GGRASDYKS | AGC | 0.0413 | up |
| YMAPYDNYV | Tyr-K | 0.0420 | up |
| LELSDDDD | CKII | 0.0422 | down |
| THVASVSDV | SnRK AMPK | 0.0423 | down |
| SMANSFVGT | PDKI | 0.0427 | down |
| DLLTSPDVG | CDC2 | 0.0441 | down |
| RGKSSSYSK | AGC | 0.0441 | up |
| SSSNTIRRP | AGC | 0.0453 | up |
| RRDSV | 0.0457 | down | |
| TKAASEKKS | 0.0469 | up | |
| DRLVSARSV | CDPK, SnRK, AGC | 0.0480 | down |
| RLSISTESQ | AMPK | 0.0489 | up |
Arabidopsis seedlings were incubated in a solution of 100 mM sucrose or sorbitol for 1 hour after which extracts were made as described before (Plos One) and incubated on Kinase1 PepChips (1152 consensus peptides spotted twice per slide; Pepscan). The averaged phosphorylation intensities obtained from three independent experiments were analyzed using a Student’s t-test. Indicated is whether phosphorylation of consensus peptides is higher (up) or lower (down) after sucrose treatment, compared to sorbitol. Kinase annotation according to Pepscan Presto (www.pepscanpresto.com), or our own analysis (see main text).