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. 2010 Mar;159(3):315–326. doi: 10.1111/j.1365-2249.2009.04071.x

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Journal Compilation © 2010 British Society for Immunology

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Fig. 1 — Detection of fractions of peripheral blood dendritic cells (PBDCs). The DC-enriched population was stained with fluorescein isothiocyanate (FITC)-labelled anti-CD11c monoclonal antibodies (mAbs), phycoerythrin (PE)-labelled mAbs against lineage markers (CD3, CD14, CD15, CD16 and CD19) (lin) and peridinin chlorophyll (PerCP)-labelled anti-human leucocyte antigen D-related (HLA-DR) mAbs. (a) DCs were detected as lin^-/HLA-DR⁺ fraction [designated as region 1 (R1)]. (b) Two fractions of DCs were identified, with phenotypes of CD11c⁺/lin^-/HLA-DR⁺ (myeloid DC; R2), CD11c^-/lin^-/HLA-DR⁺ (plasmacytoid DC; R2). Absolute numbers of PBDCs (/ml) were calculated by multiplying the percentage of lin^-/HLA-DR⁺ fraction (designated R1) within total flow cytometry events by the peripheral blood mononuclear cell (PBMC) count after negative selection (/ml).