Figure 1.

Compromised vacuolar alkalization in the root cortex cells under salt stress by inhibition of SOS1 expression in Thellungiella salsuginea. Root cells were loaded with carboxyl-SNARF, a fluorescence pH indicator whose emission spectrum shifts from 580 nm (green) to 640 nm (red) as the pH increases. Confocal images were collected at 580 nm and 640 nm separately and merged. Seedlings of five day-old Thellungiella wild type (A) and the thsos1-4 line (B) were subjected to 150 mM NaCl. Root cortex cells are shown.