Table 1.
Effect of chemotherapeutic agents on clonogenicity of breast cancer cells expressing BRMS1
| IC50 (μM) | ||||
|---|---|---|---|---|
| Drug | 231 | 231BRMS1 | 435 | 435BRMS1 |
| Doxorubicin | 0.049 (0.027–0.086) | 0.071 (0.033–0.153) | 0.122 (0.078–0.190) | 0.114 (0.065–0.199) |
| Vincristine | 0.016 (0.010–0.025) | 0.015 (0.010–0.021) | 0.001 (0.0002–0.01) | 0.001 (4.4e-006–0.472) |
| 5-Fluorouracil | 150.2 (54.06–417.4) | 151.6 (56.10–409.5) | 663 (486.7–903.1) | 476.9 (142.3–1599) |
| Paclitaxel | 0.0002 (4.5e-005–0.001) | 8.81e-005 (6.9e-006–0.001) | 0.029 (0.011–0.074) | 0.012 (0.004 to 0.034) |
231/231BRMS1 and 435/435BRMS1 cells were seeded in triplicate at a density of 1000 cells/well in 6-well plates in a final volume of 2 ml media and allowed to attach overnight. The following day, drugs were added at the indicated final concentrations in a volume of 2 ml media and allowed contact with cells for 4 h. Drug containing media was aspirated after 4 h and replaced gently with 5 ml fresh pre-warmed medium in each well. Cells were left undisturbed in a humidified incubator at 37°C for 7 days and colonies formed were counted by staining with a 1:3 solution of acetic acid:methanol containing 0.01% crystal violet (Carnoy’s fixative). Cell clusters containing >50 cells were counted as colonies. Experiments were performed in triplicate and repeated at least twice independently. Numbers in parentheses represent 95% confidence interval values. IC50 values represent concentrations of drugs resulting in a 50% reduction in colony number.