Figure 5.
Kinetics of the changes in the intensity of fluorescence of CYP3A4(C468)-BODIPY upon its interactions with liposomes. Conditions: 1 µM CYP3A4(C468)-BODIPY in 0.1 M Na-HEPES buffer, 1 mM DTT, and 1 mM EDTA. Process was initiated by the addition of the liposomes to yield the L/P ratio of 100 (circles) and 1200 (triangles). Solid lines show the results of fitting of the kinetic curves with a bi-exponential equation, which gives the maximal amplitudes of the observed changes of 26% and 78% respectively.