. Author manuscript; available in PMC: 2011 Feb 16.

Published in final edited form as: Biochemistry. 2010 Feb 16;49(6):1290. doi: 10.1021/bi9020296

Table 2.

Steady-State Kinetics Data for Proline to Alanine Tether Mutants^a

Enzyme	k_cat (s⁻¹)	K_m^sulfite (µM)	k_cat /K_m^sulfite (M⁻¹s⁻¹)
wt HSO^b	26.9 ± 0.5	11.1 ± 0.4	2.4 × 10⁶
P105A	39 ± 2	6 ± 1	6.1 × 10⁶
P111A	50 ± 3	33 ± 5	1.5 × 10⁶
P105A / P111A	46 ± 2	32 ± 3	1.5 × 10⁶

pH 8.0, 20 mM Tris acetate;