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. Author manuscript; available in PMC: 2011 Feb 1.
Published in final edited form as: J Neurochem. 2009 Dec 4;112(4):1045–1053. doi: 10.1111/j.1471-4159.2009.06528.x

Figure 3.

Figure 3

Immunocaptured BACE1 and BACE2 prefer the APPΔNL sequence over the corresponding hexameric sequence (P3′ to P3) from wild type human APP, as shown by time dependent cleavage of both substrates at 37°C. Data are expressed as base fluorescence units corrected to background (control wells, identical except with antibody omitted) at each time point. Capture at either the N- or C-terminus gave similar results, although N-terminal capture usually resulted in greater total activity. Antibodies: MAB931 (N-terminal) or MAB5308 (C-terminal) for BACE1; Ab1 (N-terminal) or Ab5670 (C-terminal) for BACE2.