Fig. 2.

CAPE blocks NF-κB activation human macrophages. (A) Representative confocal laser scanning microscopy images for nuclear translocation of the p65 subunit of NF-κB in CAPE treated cells. Monolayers of hMDMs were treated with 20 µg ml⁻¹ of CAPE 30 min prior to infection. Cells were infected with the wt strain of F. tularensis subsp. novicida at a moi 10. The parental strain AA100 of L. pneumophila was used as positive control. Uninfected and E. coli LPS-treated (1 µg ml⁻¹ for 20 min) monolayers were included as a negative and positive control respectively. (B) Quantitative analysis of p65 nuclear translocation in F. tularensis-infected macrophages in CAPE treated cells. The results are representative of three independent experiments and error bars represent standard deviations. The asterisks represent statistically significant difference.