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. Author manuscript; available in PMC: 2011 Mar 1.
Published in final edited form as: J Neurochem. 2009 Dec 9;112(5):1190–1198. doi: 10.1111/j.1471-4159.2009.06534.x

Figure 3. Fpn protein expression enhanced glutamate uptake in Mn treated HEK cells.

Figure 3

Six hour of Mn exposure (100, 200, or 500 µM) induced decrease of glutamate uptake in a concentration-dependent manner in WT HEK293T and ponasterone A uninduced HEK293T cells; increased Fpn protein expression in ponasterone A induced HEK293T-Fpn-GFP cells was associated with a reversal of the Mn-induced (100 and 250 µΜ) decrease in glutamate uptake. High level (500 µM) of Mn on glutamate uptake remained significantly decreased in induced HEK293T-Fpn-GFP cells compared with WT cells. Glutamate uptake in ponasterone A induced HEK293T-Fpn-GFP cells was significantly higher vs. WT HEK293T and ponasterone A uninduced HEK293T-Fpn-GFP cells at the same Mn treatments. Values are mean ± SEM derived from three independent experiments with three or more samples per experiment. * p<0.05, *** p<0.001 compared to control; Δ p<0.05, ΔΔΔ p<0.001 compared between treatments at the same concentration in the different groups.