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. Author manuscript; available in PMC: 2011 Feb 1.
Published in final edited form as: Gastroenterology. 2009 Oct 17;138(2):705. doi: 10.1053/j.gastro.2009.10.002

Figure 3. MeCP2 expression in HSC.

Figure 3

(a) 30μg whole cell extract from freshly isolated HSC harvested at day 1, 2, 3 and 7 of culture were separated by SDS-PAGE and immunoblotted for MeCP2 and β actin. (b) RNA isolated from qHSC (culture day 0) and myofibroblasts (culture day 7) was used for semi-quantitiative RT-PCR analysis of MeCP2 and β actin or (c) or interrogated with primers specific for short/long MeCP2 transcript.