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. Author manuscript; available in PMC: 2011 Feb 15.
Published in final edited form as: Toxicol Appl Pharmacol. 2009 Nov 17;243(1):63. doi: 10.1016/j.taap.2009.11.013

Fig. 3.

Fig. 3

U0126 prevents upregulation of Egr-1 mRNA in bile acid-treated hepatocytes. Primary mouse hepatocytes were treated with 10μM UO126 or vehicle followed by either 200μM DCA or 200μM CDCA 30 minutes later. (A) After a 15 minute incubation, phosphorylated-Erk1/2 and total-Erk1/2 were detected by western blot. Representative of an n=3. (B and C) After 2 hours, Egr-1 mRNA levels were quantified by real-time PCR. Data are expressed as mean +/- SEM; n=3. aSignificantly different (p < 0.05) from control-treated hepatocytes. bSignificantly different (p < 0.05) from hepatocytes treated with vehicle and bile acid.