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. Author manuscript; available in PMC: 2011 Feb 24.

Published in final edited form as: Vet Microbiol. 2009 Aug 8;141(1-2):12. doi: 10.1016/j.vetmic.2009.07.035

Fig. 1 — Depolymerization of microtubules inhibits EHV-1 infection. Triplicate cultures of ED cells were mock-treated or treated with increasing amounts of the microtubule depolymerizing drugs (A) nocodazole, (B) vinblastine, or (C) colchicine for 30 min at 37°C and then infected with EHV-1 (L11ΔgIΔgE) at an MOI of 5 for 5.5 h in the presence of the drugs. β-galactosidase expression was measured by an ONPG assay (filled circles). Absorbance at 405nm was measured for each sample and A_405nm values from infected cells that were not treated with drug were set as 100% infection. Cell viability at each concentration of drug was measured by an MTS assay (open circles) and viability of cells that were not treated with drug was set at 100%.

Fig. 1 — Depolymerization of microtubules inhibits EHV-1 infection. Triplicate cultures of ED cells were mock-treated or treated with increasing amounts of the microtubule depolymerizing drugs (A) nocodazole, (B) vinblastine, or (C) colchicine for 30 min at 37°C and then infected with EHV-1 (L11ΔgIΔgE) at an MOI of 5 for 5.5 h in the presence of the drugs. β-galactosidase expression was measured by an ONPG assay (filled circles). Absorbance at 405nm was measured for each sample and A_405nm values from infected cells that were not treated with drug were set as 100% infection. Cell viability at each concentration of drug was measured by an MTS assay (open circles) and viability of cells that were not treated with drug was set at 100%.