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. Author manuscript; available in PMC: 2011 Feb 15.
Published in final edited form as: Nitric Oxide. 2009 Nov 4;22(2):149. doi: 10.1016/j.niox.2009.10.009

Figure 1. Evidence of power saturation at 5K for low spin MetHb-nitrite.

Figure 1

(a) EPR peak intensities of high spin MetHb and low spin MetHb-nitrite measured at 5K using different microwave powers. MetHb-nitrite was prepared in 0.01 M PBS buffer at pH 6.5 by adding 10 mM nitrite to 200 μM MetHb. (b) Wide field EPR scans of MetHb-nitrite measured at 5K under varying microwave powers. Same samples as described for panel a. The high spin MetHb peak recorded for Figure 3a is at ~ 1124 gauss and the lowest field peak for the low spin MetHb-nitrite peak is at ~2214 gauss.