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. Author manuscript; available in PMC: 2011 Feb 15.
Published in final edited form as: Nitric Oxide. 2009 Nov 4;22(2):149. doi: 10.1016/j.niox.2009.10.009

Figure 3. EPR of MetHb and MetHb-nitrite taken at 15K and 0.8mW microwave power.

Figure 3

(a) 300uM MetHb was prepared in 0.01M PBS buffer at pH 6.5 at 0 °C. 300uM nitrite was added for a [Nit]:[Met] ratio of 1:1. (b) 6mM nitrite was added for a [Nit]:[Met] ratio of 20:1. Kd values were calculated by fitting simulations of the high spin MetHb and two low spin MetHb-nitrite species to the experimental data and calculating concentrations of each species from the simulations. The Kd value increases as the [Nit]:[Met] ratio increases.