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. Author manuscript; available in PMC: 2011 Feb 15.
Published in final edited form as: Nitric Oxide. 2009 Nov 4;22(2):149. doi: 10.1016/j.niox.2009.10.009

Figure 5. Nitrite binding to MetHb in the presence of allosteric affectors.

Figure 5

(a) Nitrite (1.5 mM) was added to MetHb (300 μM) that had been incubated with 3 mM NEM or IHP at pH 7.4. The high field EPR spectra are shown demonstrating significant differences in the shape of the spectra. (b) The dissociation constants were obtained from EPR spectra on samples as described for panel “a” where the ratio of nitrite to MetHb is 5:1 and also for when 6 mM nitrite was added (nitrite to MetHb ratio of 20:1).