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. Author manuscript; available in PMC: 2011 Feb 4.
Published in final edited form as: DNA Repair (Amst). 2009 Dec 16;9(2):109. doi: 10.1016/j.dnarep.2009.11.002

Fig. 2.

Fig. 2

(A) Immunoblotting for Pol β in siRNA knockdown (KD) XPA-vector and XPA-UVDE cells. Cell extracts were prepared from human XPA-vector cells with control siRNA (lane 1) and human XPA-UVDE cells with control siRNA (lane 2) or siRNA to Pol β (lane 3). They were immunoblotted with antibody for Pol β and PCNA as a loading control. (B) In vivo repair of SSB-bearing CPDs in siRNA knockdown XPA-UVDE and XPA-vector cells. The experiment was conducted as described in Fig. 1. (C) Relative intensities of CPD signal during the indicated repair period after 254 nm local UV exposure of 30 J/m2 in control and Pol β KD XPA-UVDE cells and XPA-vector cells.