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. Author manuscript; available in PMC: 2011 Feb 4.
Published in final edited form as: DNA Repair (Amst). 2009 Dec 16;9(2):109. doi: 10.1016/j.dnarep.2009.11.002

Fig. 9.

Fig. 9

Coordination of Pol β and FEN1 on the nicked CPD ODN substrate. (A) Schematic diagram of the reaction. The Pol β substrate was the 5′-end labeled 14-mer upstream strand and the product was expected to be observed beyond 14-mer (left side scheme). The FEN1 substrate was the 3′-end labeled 28-mer downstream strand containing CPD lesion (TT) at the 5′-end. The observed FEN1 cleavage product was expected to less than 28-mer (right side scheme). (B) Typical result of Pol β and FEN1 coordination reaction is shown. The in vitro Pol β synthesis and FEN1 cleavage reaction was simultaneously conducted using Pol β (1 nM) and FEN1 [20 nM (lane 1) and 100 nM (lane 2)]. The positions of Pol β synthesis product and FEN1 cleavage product are shown.