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. Author manuscript; available in PMC: 2011 Feb 4.
Published in final edited form as: DNA Repair (Amst). 2009 Dec 8;9(2):120. doi: 10.1016/j.dnarep.2009.11.003

Fig. 6.

Fig. 6

DNA damage-dependent colocalization of hMLH1, hMSH6, and PCNA on the chromatin as determined by immunofluorescence. HeLa cells were plated onto glass coverslips, synchronized by a DTB and treated with 10 μM MNNG and O6-BG or DMSO. The coverslips were removed at 4h (A) or 8h (B) following release from the DTB. The cells were extracted with CSK buffer to remove all soluble proteins and examined by confocal immunofluorescence with antibodies against hMLH1, hMSH6, and PCNA. Detergent resistant hMLH1 and hMSH6 are visible in MNNG-treated cells and co-localize to a modest extent. PCNA is detergent resistant in mock and MNNG-treated cells at 4 hours (A), when cells are in S-phase, however, only MNNG-treated cells contain detergent resistant PCNA at 8 hours (B). A sub-population of the chromatin-bound PCNA co-localizes with hMSH6.